Screening Mammalian Cochlear Hair Cells to Identify Critical Processes in Aminoglycoside-Mediated Damage
نویسندگان
چکیده
منابع مشابه
Lysosomal augmentation during aminoglycoside uptake in cochlear hair cells.
Aminoglycoside antibiotics, such as kanamycin, have ototoxic side effects, which often result in degeneration of cochlear and vestibular hair cells in the inner ear. Cytotoxic effects of aminoglycosides, however, do not appear immediately after cellular uptake of aminoglycosides. In order to understand the mechanisms responsible for the delayed emergence of aminoglycoside ototoxicity, changes i...
متن کاملResponse characteristics of mammalian cochlear hair cells.
Intracellular recordings were made from the low frequency region (third turn) of the guinea pig cochlea. Response characteristics are compared to gross potentials obtained from the organ of Corti fluid space. Inner hair cells (IHCs) possess relatively low (median, -32 mV) initial membrane potentials, whereas that of outer hair cells (OHCs) is higher (median, -53.5 mV). In response to tone burst...
متن کاملFunctional assembly of mammalian cochlear hair cells
Hair cells in the mammalian inner ear convert sound into electrical signals that are relayed to the nervous system by the chemical neurotransmitter glutamate. Electrical information encoding sound is then passed through the central nervous system to the higher auditory centres in the brain, where it is used to construct a temporally and spatially accurate representation of the auditory landscap...
متن کاملProtecting Mammalian Hair Cells from Aminoglycoside-Toxicity: Assessing Phenoxybenzamine’s Potential
Aminoglycosides (AGs) are widely used antibiotics because of their low cost and high efficacy against gram-negative bacterial infection. However, AGs are ototoxic, causing the death of sensory hair cells in the inner ear. Strategies aimed at developing or discovering agents that protect against aminoglycoside ototoxicity have focused on inhibiting apoptosis or more recently, on preventing antib...
متن کاملReversible contraction of isolated mammalian cochlear hair cells
Outer hair cells were isolated from the guinea pig cochlea using a micromechanical non-enzymatic procedure. Depolarization of outer hair cells in the presence of 25-125 mM K+ was accompanied by a longitudinal contraction of the isolated cells. A decrease of [K+] to 5.4 mM interrupted contraction and induced a relaxation. Individual hair cells were able to undergo as many as 5 cycles of contract...
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ژورنال
عنوان ژورنال: Frontiers in Cellular Neuroscience
سال: 2018
ISSN: 1662-5102
DOI: 10.3389/fncel.2018.00179